ParkinsonCanadaV1, Main, Exploration, bibRecord, 002663

Modulation of tyrosine hydroxylase expression by melatonin in human SH-SY5Y neuroblastoma cells

Identifieur interne : 002663 ( Main/Exploration ); précédent : 002662; suivant : 002664

Modulation of tyrosine hydroxylase expression by melatonin in human SH-SY5Y neuroblastoma cells

Auteurs : Catherine R. Mcmillan [Canada] ; Rohita Sharma [Canada] ; Tom Ottenhof [Canada] ; Lennard P. Niles [Canada]

Source :

Neuroscience letters [ 0304-3940 ] ; 2007.

RBID : Pascal:07-0338042

Descripteurs français

Pascal (Inist)
- Tyrosine 3-monooxygenase, Mélatonine, Neurone, Lignée cellulaire, Récepteur mélatonine MT1, Protéine, Récepteur biologique, Différenciation, Rétinoïque acide, Homme, Lignée SHSY5Y.
Wicri :
- topic : Homme.

English descriptors

KwdEn :
- Biological receptor, Cell line, Differentiation, Human, MT1 melatonin receptor, Melatonin, Neuron, Protein, Retinoic acid, Tyrosine 3-monooxygenase.

Abstract

We have previously reported in vivo preservation of tyrosine hydroxylase (TH), the rate-limiting enzyme in dopamine synthesis, following treatment with physiological doses of melatonin, in a 6-hydroxydopamine model of Parkinson's disease. Based on these findings, we postulated that melatonin would similarly modulate the expression of TH in vitro. Therefore, using human SH-SY5Y neuroblastoma cells which can differentiate into dopaminergic neurons following treatment with retinoic acid, we first examined whether these cells express melatonin receptors. Subsequently, the physiological dose-dependent effects of melatonin on TH expression were examined in both undifferentiated and differentiated cells. The novel detection of the G protein-coupled melatonin MT₁ receptor in SH-SY5Y cells by RT-PCR was confirmed by sequencing and Western blotting. In addition, following treatment of SH-SY5Y cells with melatonin (0.1-100 nM) for 24 h, Western analysis revealed a significant increase in TH protein levels. A biphasic response, with significant increases in TH protein at 0.5 and 1 nM melatonin and a reversal at higher doses was seen in undifferentiated cells; whereas in differentiated cells, melatonin was effective at doses of 1 and 100 nM. These findings suggest a physiological role for melatonin in modulating TH expression, possibly via the MT₁receptor.

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">We have previously reported in vivo preservation of tyrosine hydroxylase (TH), the rate-limiting enzyme in dopamine synthesis, following treatment with physiological doses of melatonin, in a 6-hydroxydopamine model of Parkinson's disease. Based on these findings, we postulated that melatonin would similarly modulate the expression of TH in vitro. Therefore, using human SH-SY5Y neuroblastoma cells which can differentiate into dopaminergic neurons following treatment with retinoic acid, we first examined whether these cells express melatonin receptors. Subsequently, the physiological dose-dependent effects of melatonin on TH expression were examined in both undifferentiated and differentiated cells. The novel detection of the G protein-coupled melatonin MT<sub>1</sub>
 receptor in SH-SY5Y cells by RT-PCR was confirmed by sequencing and Western blotting. In addition, following treatment of SH-SY5Y cells with melatonin (0.1-100 nM) for 24 h, Western analysis revealed a significant increase in TH protein levels. A biphasic response, with significant increases in TH protein at 0.5 and 1 nM melatonin and a reversal at higher doses was seen in undifferentiated cells; whereas in differentiated cells, melatonin was effective at doses of 1 and 100 nM. These findings suggest a physiological role for melatonin in modulating TH expression, possibly via the MT<sub>1</sub>
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Modulation of tyrosine hydroxylase expression by melatonin in human SH-SY5Y neuroblastoma cells

Modulation of tyrosine hydroxylase expression by melatonin in human SH-SY5Y neuroblastoma cells

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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